Dylight 550 vs pe

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August undefined, 2024

WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View … WebR-PE & APC Labeled Antibodies; Biotin & Streptavidin Conjugates; ... DyLight® 350. Features. Brighter and more photostable than AMCA ... 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine. Features. Brighter than Cy®3 Highly water-soluble Validated in multicolor STORM super-resolution imaging.

Fluorochrome Guide - Leinco Technologies

WebBackground. CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface … WebCatalog number: 62262. Thermo Scientific DyLight 550 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight … highly sensitive crp cardiac

Lightning-Link® Rapid DyLight 550 Antibody Labeling Kit

WebDyLight 550 561 562 576 FL3 FL2 FL2 DyLight 550 PE 488, 561 496, 565 578 FL3 FL3 FL2 FL2 FL2 FL2 PE ... PE-Cy5 488, 561 496, 565 670 FL4 FL4 FL4 FL4 FL3 FL2 PE … WebDyLight™ 550 has a bright orange to red fluorescence rhodamine derivative dye. This dye can be used to replace Alexa555, TRITC and Cy3. DyLight™ 550 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross ... WebH9 human embryonic stem (ES) cells were stained with either PE Mouse Anti-SSEA-4 (solid line) or PE mouse IgG3 (A112-3) isotype control (catalog number 559926, dashed line), incubated in the dark for 20 minutes at room temperature and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur™ System. small room conference system

Antibodies Conjugated to Fluorophores Bio-Rad

Is Alexa Fluor more suitable than DyLight? ResearchGate

WebView Mouse Monoclonal anti-HLA DRB1 Antibody (SPM423) [PE] (NBP3-11552PE). Validated Applications: Flow. Validated Species: Human, Monkey. WebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. ... with the exception of RPE and PE tandem dyes. For more details on the recommendations … small room coolers reviewsWebMouse Anti-DYKDDDDK IgG: DyLight® 550;100ug quantity ... Mouse Anti-DYKDDDDK IgG: SureLight® PE-665;100ug. Clone: M2 $ 378.00. #D14-1718. Add to cart Details; Mouse anti-DYKDDDDK IgG: Alkaline Phosphatase;100ug. Clone: M2 Recommended dilution of 1:500 – 1:10000. Starting dilution: 1:2000. small room computer table

"WebDyLight 550 is brighter in most applications than TRITC, Cy3 & AlexaFluor 555. All Dylight 550 ... R-PE (Ex Max 496 nm / Em. Peak 578nm) R-phycoerythrin (R-PE) has a … " - Dylight 550 vs pe

Dylight 550 vs pe

Frequently asked questions - DyLight® 550 repla

WebJan 10, 2024 · TRITC is excited with light in the green spectrum with a maximum at 550 nm. Its emission maximum is lying at 573 nm. The bond to proteins (e.g. antibodies) is also based on a reactive isothiocyanate group. ... DyLight 405: 399 nm: 434 nm: DyLight 350: 332 nm: 435 nm: BFP (Blue Fluorescent Protein) 380 nm: 439 nm: Alexa 350: 343 nm: … WebLDL is the major carrier of cholesterol in the blood, accounting for more than 60% of total plasma cholesterol. LDL is taken up by hepatic and extra-hepatic tissues through receptor mediated endocytosis triggered by apoB-100-LDL receptor interaction. The internalized LDL particle is transported to lysosomes where it is degraded to free ...

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WebDyLight 550 562 576 4 PE* 496, 546 578 5 APC 650 661 4 Alexa Fluor 647 650 665 4 DyLight 650 654 673 4 PerCP 490 675 2 Alexa Fluor 700 Infrared 702 723 2 * PE is the … Web10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. Labeling Reaction . 1. To protect reagents from moisture, allow DyLight NHS Esters and DMF to equilibrate to room temperature before opening vials. 2.

WebTable of Fluorochromes. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Web38 rows · PE/Cyanine5: 565: 670: 660/20: 5: PE/Dazzle™ 594: 565: 610: 610/20: 5: PE/Fire™ 700* 565: 695: 670/30: 5: Brilliant Violet 421™ ... Spark Blue™ 550* 516: 540 …

WebGoat IgG (2mg/mL) was reacted with a 15-fold molar excess of DyLight 550 NHS Ester (Part No. 62262). A. Measure A. 280. and A. max. of the DyLight 550-conjugated Goat IgG . 1. The final labeling reaction solution was dialyzed overnight to remove excess, nonconjugated dye. 2. The dialyzed conjugate was diluted 1/10 in phosphate buffered … WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View …

WebThermo Scientific DyLight 550 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 550 used to fluorescently label sulfhydryl-containing …

WebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. DyLight dyes are extremely bright and are highly water soluble meaning that a high dye-protein ratio ... highly sensitive or autisticWebDyLight® Dyes. DyLight® dyes give you outstanding immunofluorescence results in flow cytometry and microscopy. Newborn rat primary cardiac myocytes stained with mouse anti-desmin, detected with goat anti-mouse IgG:DyLight® 488 (STAR74D488); rat anti-alpha tubulin detected with goat anti-rat IgG:DyLight® 649 (STAR71D649); and rabbit anti ... small room cooling solutionsWebHiLyte FluorTM 555 550 566 5-TAMRA 541 568 Alexa Fluor®546 556 573 532 DyLight®550 562 576 Phycoerythrin (PE) 496,566 576 488 Tetramethyl Rhodamine Isothiocyanate (TRITC) 557 576 EviTag TM quantum dots-Birch Yellow 560 580 Cy3.5® … highly sensitive intpWeb62269 DyLight 550-2xPEG NHS Ester, 1mg. 62274 DyLight 650-4xPEG NHS Ester, 1mg. 46601 DyLight 680-4xPEG NHS Ester, 1mg. 62277 DyLight 755-4xPEG NHS Ester, … small room covingWebBoth the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation. Our catalog includes secondary antibodies … small room circulation fansWebBoster Bio Anti-Human CD2AP DyLight® 550 conjugated Antibody catalog # A01756-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human. … small room couchWebYes, the same experimental set-up can be used. 4. What is the excitation and emission of the DyLight® 650 fluorochrome? The excitation is 652nm and the emission is 672nm. As a comparison, the excitation of DyLight® 649 was 654nm and the emission was 673nm. 5. small room cooler