Dynamics of gene expression in single
WebApr 6, 2024 · Gene expression is the process by which cells produce proteins from information stored in DNA. Due to the small number of molecules involved in this process and their short lifetimes, random fluctuations or noise in protein levels are an inherent feature of gene expression. Single-cell studies have shown that stochasticity in gene … WebTo decipher cell state transitions from single-cell transcriptomes it is crucial to quantify weak expression of lineage-determining factors, which requires computational methods that are sensitive to the variability of weakly expressed genes. ... Revealing dynamics of gene expression variability in cell state space Nat Methods. 2024 Jan;17(1 ...
Dynamics of gene expression in single
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Web7 hours ago · The upregulated expression gene, the response to dehydration 29A (AtRD29A), regarded as an indicator ... Lateral root formation at different developmental … Web7 hours ago · Introductionβ-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied.MethodsBased on cloned the mannanase (MAN) gene from Mirabilis jalapa L., …
Webearly embryo, new gene expression generally arises in single contiguous domains. To askwhether the single-cell gene expression states conform to a branching pattern and, if … WebJun 11, 2024 · Gene expression programs are dynamic, e.g. the cell cycle, response to stimuli, normal differentiation and development, etc. However, nearly all techniques for …
WebThe underlying mechanisms that determine gene expression and chromatin accessibility in retinogenesis are poorly understood. Herein, single-cell RNA sequencing and single … WebMay 11, 2024 · By modelling gene expression as a Gaussian process, two competing models of single cell dynamics were proposed. An OU model describes random aperiodic fluctuations, but in contrast to white noise the fluctuations are correlated over time, and this may form a more appropriate model of non-oscillating single cells for statistical testing.
WebJul 18, 2024 · Here we examine an established population of human adipose-derived stem cells (ASCs) using a novel, microfluidic-based method for high-throughput transcriptional …
WebApr 14, 2024 · The determination of the gene clusters revealed the correspondence between RNA kinetics and gene functions. This is indeed a remarkable and groundbreaking method, as it will now aid researchers in probing intricate questions concerning the dynamics of the transcriptome and gene expression regulation. great western boot repairWebOct 1, 2011 · Imaging gene expression in single cells The heterogeneous nature of gene expression on the cellular level has been observed in the microscope for several decades. Early studies using β-galactosidase as a fluorogenic readout for lacZ expression indicated that when the lacZ reporter gene was driven off of the promoter of interest, one observed ... florida mold assessorWebAug 15, 2008 · Here, we are interested in the dynamics of such a switching process on the single cell level. We study these dynamics in the context of the arabinose utilization (ara) system of E. coli (11), another well-characterized bistable system (see Fig. 1).In this case, arabinose is imported by the high-affinity low-capacity transporter AraFGH and the low … great western brewery original 16WebOct 26, 2024 · The use of the MS2-MCP approach to monitoring multiple steps of the RNA life cycle in real-time has provided unprecedented insights into the process of gene expression . Analysis of protein turnover by fluorescent probes was widely performed to elucidate gene expression as well (22, 23). However, to explore chromatin dynamics … florida mold laws in condosWebBy using cap analysis of gene expression (CAGE), we mapped transcription initiation events at single nucleotide resolution across 12 stages of zebrafish development. These CAGE-based transcriptome maps reveal genome-wide rules of core promoter usage, structure, and dynamics, key to understanding the control of gene regulation during … florida modular beach homesWebApr 14, 2024 · The determination of the gene clusters revealed the correspondence between RNA kinetics and gene functions. This is indeed a remarkable and … florida mold certification coursesWebJan 10, 2014 · To investigate allele-specific gene expression at single-cell resolution, we isolated 269 individual cells dissociated from in vivo F1 embryos (CAST/EiJ × C57BL/6J, hereafter abbreviated as CAST and C57, respectively) from oocyte to blastocyst stages of mouse preimplantation development (PD) ().We generated transcriptome profiles with … florida mold inspection certification