Phi29 polymerase structure
WebMay 19, 2024 · Phi29 DNA polymerase (Phi29 Pol) has been successfully applied in DNA nanoball-based sequencing, real-time DNA sequencing from single polymerase molecules and nanopore sequencing employing the sequencing by synthesis (SBS) method. WebMay 15, 2007 · PubMed Abstract: Replicative DNA polymerases (DNAPs) move along template DNA in a processive manner. The structural basis of the mechanism of translocation has been better studied in the A-family of polymerases than in the B-family of replicative polymerases. To address this issue, we have determined the X-ray crystal …
Phi29 polymerase structure
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WebPhi29 DNA Polymerase is normally responsible for the replication of the Bacillus subtilisphage phi291. The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity2and a 3′→ 5′ proofreading exonuclease function3. WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide …
WebJan 29, 2024 · ϕ29 DNA polymerase is the only member of the protein-primed subgroup of DNA polymerases whose structure has been solved. It has a N-terminal domain (residues … WebPolbase - Polymerase: Phi29 Phi29 From Bacteriophage φ29 (aliases:φ29) N C Mutation Position Missing mutants? 2PZS Berman AJ2007 Phi29 is a family B enzyme from Bacteriophage φ29 . References (146) Structures (8) Sequences (2) Mutants (19)... External links Search this table: Showing 1 to 146 of 146 entries
WebJul 25, 2007 · To address this issue, we have determined the X-ray crystal structures of phi29 DNAP, a member of the protein-primed subgroup of the B-family of polymerases, … WebSep 29, 2024 · DNA replication of phi29 and related phages takes place via a strand displacement mechanism, a process that generates large amounts of single-stranded …
WebMay 16, 2007 · Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex) PDB DOI: 10.2210/pdb2PYL/pdb NDB: PD1012 Classification: REPLICATION, TRANSFERASE/DNA Organism (s): Salasvirus phi29 Expression System: Escherichia coli Mutation (s): Yes Deposited: 2007-05-16 Released: …
• Linck L, Resch-Genger U (2010). "Identification of efficient fluorophores for the direct labeling of DNA via rolling circle amplification (RCA) polymerase φ29". Eur J Med Chem. 45 (12): 5561–6. doi:10.1016/j.ejmech.2010.09.005. PMID 20926164. • de Vega M, Lázaro JM, Mencía M, Blanco L, Salas M (2010). "Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs". Proc Natl Acad Sci U S A. 107 (38): 16506–11. Bibcode:2010PNAS..10716506D. common used discount codesWebFAQ. Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. … duck donuts latham nyWebPhi29 DNA polymerase uses the OH group of a specific serine residue as a primer bypassing the need for a primase. During the extension stage the phi29 DNA polymerase continues to synthesize DNA in a standard DNA-primed fashion and the displaced single-stranded regions are covered by SSB. duck donuts order form printableWebJul 24, 2024 · Herein, Phi29 DNA polymerase plays two important roles as exonuclease and polymerase. Long-stranded genomic DNAs can be broken into small DNA fragments after ultrasonication. The fragments that contain target DNA, hybridize with a linear padlock probe to trigger the formation of a circular RCA template. duck donuts in winston salemWebOct 29, 2024 · The RCA reaction relies on a DNA polymerase, phi29, which has additional 5′-3′ exonuclease activity in addition to polymerase activity. As a consequence, phi29 is capable of DNA chain ... common used diabetic management programsWebThe structure of the phi29 DNA polymerase, so far the only experimentally determined 3D structure of a protein-primed DNA polymerase, has been instrumental in understanding … common used french wordsWebApr 1, 2002 · The results suggest that Lys371 of phi29 DNA polymerase, highly conserved among families A and B, interacts with the phosphate groups of the incoming nucleotide. On the other hand, the role of residue Ile364 seems to be structural, being important for both DNA and dNTP binding. Pre-motif B must therefore play an important role in binding the ... common use for biaxin